General description

       FREY agar is fortified with nicotinamide adenine dinucleotide (NAD⁺) to satisfy the fastidious growth requirements of Mycoplasma synoviae. Metabolic activity of viable mycoplasmas produces acidic by-products that trigger a phenol-red pH indicator shift from red to yellow—or, under alkaline drift, to purple—thereby providing an immediate, visual confirmation of proliferation. The inclusion of penicillin G selectively suppresses contaminating bacterial flora without impairing mycoplasma growth.         In addition, the medium can be further enriched with a defined lipid supplement from yolk that supplies bioavailable cholesterol and fatty-acid precursors, markedly enhancing colony-forming efficiency—particularly for lipid-dependent strains such as M. pneumoniae ATCC 23714.

Application

       FREY Agar is recommended for the detection of avian mycoplasmas in pharmaceutical products, especially in bulk vaccines, cell banks, and virus cultures.

Features and Benefits

• Ready-to-use convenience as no further preparation steps are required.


• Pharmaceutical products such as Biopharmaceuticals, Vaccines, Cell cultures, and Virus cultures.

• Can be stored at 2–8 °C for up to 3 months  or -20℃ for 6 months.


• A colorimetric chart enables a rapid and convenient visual indication of early-stage mycoplasma proliferation.


• By using a buffered system, higher colony counts can be achieved while reducing the risk of mycoplasma cell death.


• The application of a chromogenic reagent that selectively stains mycoplasma enables enhanced visualization of color-shifted cells under microscopy, thereby improving detection clarity and specificity.


• To prevent growth inhibition caused by the degradation of NAD⁺ during later stages, alternative NAD⁺ analogs are co-supplemented in the culture system.